5G58
Crystal structure of A190T mutant of human hippocalcin AT 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 |
| Unit cell lengths | 50.660, 50.660, 283.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.870 - 2.540 |
| R-factor | 0.21708 |
| Rwork | 0.215 |
| R-free | 0.24642 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.546 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.870 | 2.600 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.090 | 0.680 |
| Number of reflections | 13592 | |
| <I/σ(I)> | 8.7 | 1.9 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M SODIUM CITRATE TRIBASIC DIHYDRATE PH 5.5, 18% V/V 2-PROPANOL, 16% W/V PEG 4000 |
