5G54
The crystal structure of light-driven chloride pump ClR at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-05-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.725, 49.434, 77.027 |
| Unit cell angles | 90.00, 131.16, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.17845 |
| Rwork | 0.176 |
| R-free | 0.22519 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3x3b |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.990 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.670 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.330 |
| Number of reflections | 19868 | |
| <I/σ(I)> | 14.53 | 5.15 |
| Completeness [%] | 99.2 | 97.3 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.5 |
