5G4Z
Structural basis for carboxylic acid recognition by a Cache chemosensory domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-19 |
Detector | ADSC CCD |
Spacegroup name | P 64 |
Unit cell lengths | 73.428, 73.428, 74.923 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 63.590 - 1.980 |
R-factor | 0.15785 |
Rwork | 0.157 |
R-free | 0.17741 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5g4y |
RMSD bond length | 0.020 |
RMSD bond angle | 1.972 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.500 | 2.030 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.090 | 0.880 |
Number of reflections | 16058 | |
<I/σ(I)> | 18.7 | 3.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.1 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PROTEIN AT 8.5 MG/ML IN 50 MM BISTRIS CHLORIDE, PH 6.5, 50 MM SODIUM CHLORIDE IN EQUAL VOLUME WITH 27% PEG 4000 AND 3% SUCROSE AT 20 C IN SITTING DROP PLATES. CRYSTALS WERE LATER SOAKED IN RESERVOIR SOLUTION WITH 40 MM NEUTRALIZED PROPIONATE. |