5G4V
Association of four two-k-turn units based on Kt-7 3bG,3nC, forming a square-shaped structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.650, 70.640, 92.820 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 30.493 - 2.870 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.27380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bw0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.718 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.490 | 2.940 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmerge | 0.090 | 1.420 |
| Number of reflections | 16952 | |
| <I/σ(I)> | 9.3 | 0.9 |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M AMMONIUM ACETATE, 0.01 M CA CHLORIDE, 0.05 M SODIUM CACODYLATE PH 6.5, 10% W/V POLYETHYLENE GLYCOL 4000 |
