5G49

Crystal structure of the Arabodopsis thaliana histone-fold dimer L1L NF-YC3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	68.344, 81.691, 95.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.845 - 2.300
R-factor	0.1637
Rwork	0.162
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4csr
RMSD bond length	0.008
RMSD bond angle	0.939
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.420	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.500
Number of reflections	12288
<I/σ(I)>	20.9	5.1
Completeness [%]	100.0	100
Redundancy	8.7	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				10% W/V PEG 8,000, 100 MM HEPES-NAOH PH 7.5, 200 MM CALCIUM ACETATE