5G47
Structure of Gc glycoprotein from severe fever with thrombocytopenia syndrome virus in the trimeric postfusion conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-04-18 |
| Detector | DECTRIS PILATUS3 M6 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 147.360, 152.270, 160.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.511 - 2.450 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.23200 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 108.510 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.100 | 1.080 |
| Number of reflections | 66293 | |
| <I/σ(I)> | 15.7 | 1.8 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 8.1 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 45% PENTAERYTHRIOTOL 426, 0.1 M SODIUM ACETATE AT PH 4.6 |
