5G35
Structure of Rad14 in complex with acetylaminopyren-C8-guanine containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-18 |
Detector | DECTRIS PILATUS |
Spacegroup name | P 41 |
Unit cell lengths | 53.170, 53.170, 131.307 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.170 - 2.000 |
R-factor | 0.21911 |
Rwork | 0.217 |
R-free | 0.25421 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a3d |
RMSD bond length | 0.016 |
RMSD bond angle | 1.978 |
Data reduction software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.600 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 1.250 |
Number of reflections | 24353 | |
<I/σ(I)> | 7.2 | 1 |
Completeness [%] | 99.0 | 99 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |