5G34
Structure of Rad14 in complex with acetylaminoanthracene-C8-guanine containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-30 |
| Detector | DECTRIS EIGER |
| Spacegroup name | P 41 |
| Unit cell lengths | 53.065, 53.065, 130.323 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.120 - 1.900 |
| R-factor | 0.21849 |
| Rwork | 0.217 |
| R-free | 0.24392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a3d |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.987 |
| Data reduction software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.190 | 0.680 |
| Number of reflections | 28204 | |
| <I/σ(I)> | 9.8 | 1.4 |
| Completeness [%] | 100.0 | 98 |
| Redundancy | 4.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
