5G32

Structure of Rad14 in complex with acetylaminophenyl-guanine containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-05
Detector	DECTRIS PILATUS
Wavelength(s)	0.979
Spacegroup name	P 41
Unit cell lengths	52.964, 52.964, 130.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.960 - 2.200
R-factor	0.21981
Rwork	0.218
R-free	0.26552
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.705
Data reduction software	XDS
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.160	1.110
Number of reflections	18268
<I/σ(I)>	6.62
Completeness [%]	100.0	100
Redundancy	6.8	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1