5G2Z
Crystallographic structure of mutant W31A of thioredoxin from Litopenaeus vannamei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-18 |
| Detector | RIGAKU-MSC R-AXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.156, 62.675, 99.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.429 - 1.880 |
| R-factor | 0.1895 |
| Rwork | 0.184 |
| R-free | 0.23640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zzx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.430 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.040 | 0.210 |
| Number of reflections | 17415 | |
| <I/σ(I)> | 17.6 | 4.6 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 30% (W/V) POLYETHYLENE GLYCOL 3, 000. 100 MM TRIS BASE/HYDROCHLORIC ACID PH 7.0. 200 MM NACL |
