5G1L
A double mutant of DsbG engineered for denitrosylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.650, 57.030, 85.550 |
| Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
| Resolution | 43.138 - 1.700 |
| R-factor | 0.1705 |
| Rwork | 0.168 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v57 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.956 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.120 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 61466 | |
| <I/σ(I)> | 16.73 | 2.8 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 3.3 | 2.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM CITRATE PH 3.75, 0.2 M AMMONIUM SULFATE, 20% PEG 4000 |
