5G02
Crystal Structure of zebrafish Protein Arginine Methyltransferase 2 with SFG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 147.994, 147.994, 127.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.293 - 2.451 |
| R-factor | 0.1921 |
| Rwork | 0.190 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.080 | 1.200 |
| Number of reflections | 19847 | |
| <I/σ(I)> | 11.8 | 1.1 |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 6.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 10% PEG20000, 100MM LICL,100MM MES PH6.5 |
