5FZR

Designed TPR Protein M4N delta C (CF I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-22
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	49.559, 50.858, 83.921
Unit cell angles	90.00, 102.36, 90.00

Refinement procedure

Resolution	38.252 - 2.045
R-factor	0.2199
Rwork	0.217
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fzq
RMSD bond length	0.003
RMSD bond angle	0.560
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	2.170
High resolution limit [Å]	2.050	2.050
Rmerge	0.060	0.710
Number of reflections	25655
<I/σ(I)>	18.4	2.1
Completeness [%]	98.4	94.1
Redundancy	5.58	5.69

Crystallization Conditions

crystal ID	method	pH	temperature	details
1