5FZP
Structure of the dispase autolysis inducing protein from Streptomyces mobaraensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Collection date | 2014-06-13 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.010, 101.130, 114.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.938 - 1.700 |
| R-factor | 0.1609 |
| Rwork | 0.159 |
| R-free | 0.19130 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.815 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.300 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.320 |
| Number of reflections | 148865 | |
| <I/σ(I)> | 15.56 | 3.02 |
| Completeness [%] | 98.7 | 98.9 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15% (V/V) GLYCEROL, 8.5% (V/V) 2-PROPANOL, 85 MM HEPES PH 7.5 AND 17% (W/V) PEG 4K WITH 10 MG/ML DAIP PROTEIN (IN WATER) |
