5FZP
Structure of the dispase autolysis inducing protein from Streptomyces mobaraensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Collection date | 2014-06-13 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.010, 101.130, 114.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.938 - 1.700 |
R-factor | 0.1609 |
Rwork | 0.159 |
R-free | 0.19130 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 0.815 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | HKL2MAP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.300 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.040 | 0.320 |
Number of reflections | 148865 | |
<I/σ(I)> | 15.56 | 3.02 |
Completeness [%] | 98.7 | 98.9 |
Redundancy | 2.7 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 15% (V/V) GLYCEROL, 8.5% (V/V) 2-PROPANOL, 85 MM HEPES PH 7.5 AND 17% (W/V) PEG 4K WITH 10 MG/ML DAIP PROTEIN (IN WATER) |