5FYL
Crystal Structure at 3.7 A Resolution of Fully Glycosylated HIV-1 Clade A BG505 SOSIP.664 Prefusion Env Trimer in Complex with Broadly Neutralizing Antibodies PGT122 and 35O22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-08-23 |
Detector | MARRESEARCH |
Spacegroup name | P 63 |
Unit cell lengths | 129.780, 129.780, 313.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.998 - 3.100 |
R-factor | 0.2537 |
Rwork | 0.250 |
R-free | 0.30720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tvp |
RMSD bond length | 0.005 |
RMSD bond angle | 0.961 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.100 |
Rmerge | 0.060 |
Number of reflections | 53617 |
<I/σ(I)> | 4.82 |
Completeness [%] | 85.1 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 0.18 M LI2SO4, 4.79% PEG 1500, 14.4% ISOPROPANOL, 10 MM YTTRIUM CHLORIDE, 0.09 M SODIUM ACETATE PH 5.5 |