5FYI

Crystal structure of human JMJD2A in complex with pyruvate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-07
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	100.450, 148.830, 57.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.767 - 2.096
R-factor	0.1984
Rwork	0.197
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a7o
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	DIMPLE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.770	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.050	0.680
Number of reflections	51046
<I/σ(I)>	18.9	2.3
Completeness [%]	99.9	99.2
Redundancy	6.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1M BIS-TRIS PH 6.0 -- 0.2M AMMONIUM SULFATE -- 28% PEG3350