5FYH
Crystal structure of human JMJD2A in complex with fumarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-07 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.840, 149.130, 56.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.798 - 2.348 |
R-factor | 0.2044 |
Rwork | 0.202 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a7o |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | DIMPLE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.830 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.070 | 0.820 |
Number of reflections | 36606 | |
<I/σ(I)> | 16.8 | 2.2 |
Completeness [%] | 99.8 | 98.7 |
Redundancy | 6.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 0.1M BIS-TRIS PH 6.0 -- 0.15M AMMONIUM SULFATE -- 22% PEG3350 |