5FXU
Crystal Structure of Puumala virus Gn glycoprotein ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-17 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.570, 66.790, 77.350 |
| Unit cell angles | 107.30, 93.55, 100.94 |
Refinement procedure
| Resolution | 73.260 - 2.280 |
| R-factor | 0.19015 |
| Rwork | 0.189 |
| R-free | 0.21939 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.617 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.110 | 0.820 |
| Number of reflections | 43115 | |
| <I/σ(I)> | 12.1 | 2 |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 5.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 30% W/V POLYETHYLENE GLYCOL 6000 AND 0.1 M CITRATE BUFFER PH 5.0 |
