5FXP
Crystal structure of eugenol oxidase in complex with vanillin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-10 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.920, 97.450, 180.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.110 - 2.600 |
| R-factor | 0.18392 |
| Rwork | 0.180 |
| R-free | 0.26163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vao |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.545 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.160 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.140 | 0.420 |
| Number of reflections | 29395 | |
| <I/σ(I)> | 7.7 | 2.2 |
| Completeness [%] | 91.6 | 80.4 |
| Redundancy | 3.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 24% PEG6000, 0.1 M TRIS/HCL PH 8.0 |
