5FXD
Crystal structure of eugenol oxidase in complex with isoeugenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-31 |
| Detector | PILATUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.720, 96.690, 179.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.860 - 1.700 |
| R-factor | 0.16172 |
| Rwork | 0.160 |
| R-free | 0.19881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vao |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.988 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.120 | 0.990 |
| Number of reflections | 109694 | |
| <I/σ(I)> | 7.5 | 1.8 |
| Completeness [%] | 98.7 | 98 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 24% PEG6000, 0.1 M TRIS/HCL PH 8.0 |
