5FX0
Fasciola hepatica calcium binding protein FhCaBP2: Structure of the dynein light chain-like domain. P6422 native.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 59.630, 59.630, 90.062 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.640 - 2.300 |
| R-factor | 0.20938 |
| Rwork | 0.202 |
| R-free | 0.27491 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fwz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.452 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 5253 | |
| <I/σ(I)> | 22.7 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 10 ML OF 10 MM TRIS-HCL PH 8.5, 50 MM SODIUM CHLORIDE, 20 % (W/V) PEG 3350, 0.2 M SODIUM TARTRATE |
