5FWS
Wnt modulator Kremen crystal form I at 1.90A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-10 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.940, 50.940, 188.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.800 - 1.900 |
| R-factor | 0.18149 |
| Rwork | 0.179 |
| R-free | 0.22658 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.050 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.100 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.150 |
| Number of reflections | 23300 |
| <I/σ(I)> | 11.4 |
| Completeness [%] | 99.8 |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 2.0 M AMMONIUM SULFATE, 5 %V/V ISO-PROPANOL, pH 7.5 |
