5FWI
structure of usp7 catalytic domain and three ubl-domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 115.183, 195.040, 219.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 109.890 - 3.400 |
| R-factor | 0.22436 |
| Rwork | 0.222 |
| R-free | 0.26693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1NB8 2ylm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.278 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.060 | 0.730 |
| Number of reflections | 17198 | |
| <I/σ(I)> | 13.4 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15% PEG 3350 0.2M NA CITRATE |
