5FWH
N-terminal FHA domain from EssC a component of the bacterial Type VII secretion apparatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.792, 49.887, 94.162 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.080 - 2.060 |
| R-factor | 0.24097 |
| Rwork | 0.237 |
| R-free | 0.31307 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.999 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.170 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.040 | 0.450 |
| Number of reflections | 12092 | |
| <I/σ(I)> | 27.3 | 3.6 |
| Completeness [%] | 99.1 | 94.4 |
| Redundancy | 11.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
