5FV6
KpFlo11 presents a novel member of the Flo11 family with a unique recognition pattern for homophilic interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.440, 58.680, 85.270 |
| Unit cell angles | 90.00, 96.56, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.22505 |
| Rwork | 0.223 |
| R-free | 0.26673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.541 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.520 |
| Number of reflections | 24319 | |
| <I/σ(I)> | 10 | 2.9 |
| Completeness [%] | 97.5 | 98.3 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1 M SODIUM ACATATE PH 4.6; 12 % PEG 4000 |
