5FUK
Crystallization of a dimeric heme peroxygenase from the fungus Marasmius rotula
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-28 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.010, 76.670, 120.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.360 - 1.550 |
| R-factor | 0.15628 |
| Rwork | 0.155 |
| R-free | 0.18297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fuj |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.061 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.040 | 0.370 |
| Number of reflections | 79844 | |
| <I/σ(I)> | 16.67 | 2.1 |
| Completeness [%] | 98.5 | 92.6 |
| Redundancy | 3.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN WAS CRYSTALLIZED IN 18% PEG 4000, 15% GLYCEROL. |
