5FUF
Crystal structure of the Mep2 mutant S453D from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-03 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 109.398, 109.398, 341.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.534 - 2.066 |
| R-factor | 0.1621 |
| Rwork | 0.162 |
| R-free | 0.17430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5aez |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.851 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.070 | 0.700 |
| Number of reflections | 48392 | |
| <I/σ(I)> | 17.1 | 2 |
| Completeness [%] | 99.3 | 91.5 |
| Redundancy | 10.9 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 24% PEG400 0.05 M SODIUM ACETATE PH 5.4 0.05 M MAGNESIUM ACETATE TETRAHYDRATE 10 MM AMMONIUM CHLORIDE |
