5FU7
drosophila nanos NBR peptide bound to the NOT module of the human CCR4-NOT complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.950, 135.670, 104.970 |
| Unit cell angles | 90.00, 107.98, 90.00 |
Refinement procedure
| Resolution | 48.580 - 3.100 |
| R-factor | 0.168 |
| Rwork | 0.165 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c0d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.170 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 36658 | |
| <I/σ(I)> | 13 | 1.7 |
| Completeness [%] | 99.6 | 98.2 |
| Redundancy | 3.45 | 3.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M MES PH 6.0, 260 MM LICL, 18.6 % PEG 6000 |
