5FU5
The complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-06 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 69.908, 69.908, 123.064 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.540 - 1.500 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.22122 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.571 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCDE |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.160 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 1.200 |
| Number of reflections | 29090 | |
| <I/σ(I)> | 18.3 | 2.3 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 10.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM CITRIC ACID PH 3.5 2.0M AMMONIUM SULFATE |
