5FTZ
AA10 lytic polysaccharide monooxygenase (LPMO) from Streptomyces lividans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.620, 32.430, 61.320 |
| Unit cell angles | 90.00, 97.77, 90.00 |
Refinement procedure
| Resolution | 60.760 - 1.380 |
| R-factor | 0.13634 |
| Rwork | 0.135 |
| R-free | 0.15999 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bem |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.721 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.900 | 1.400 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.060 | 0.680 |
| Number of reflections | 27360 | |
| <I/σ(I)> | 14.8 | 1.8 |
| Completeness [%] | 97.3 | 92.2 |
| Redundancy | 4.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.05 M SODIUM ACETATE PH 4.6, 25 % PEG 4,000 |
