5FTY

Structure of surface layer protein SbsC, domains 6-7 (monoclinic form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	54.668, 48.988, 91.812
Unit cell angles	90.00, 99.86, 90.00

Refinement procedure

Resolution	37.987 - 2.600
R-factor	0.2698
Rwork	0.268
R-free	0.30730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uj8
RMSD bond length	0.003
RMSD bond angle	0.646
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.200	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.550
Number of reflections	16697
<I/σ(I)>	15.5	2.3
Completeness [%]	99.0	97.8
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1