5FTX

Structure of surface layer protein SbsC, domains 4-9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 2M
Spacegroup name	C 1 2 1
Unit cell lengths	100.627, 48.128, 196.136
Unit cell angles	90.00, 96.60, 90.00

Refinement procedure

Resolution	33.038 - 4.100
R-factor	0.2061
Rwork	0.203
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 4UJ6 4uic 4UIE AND 4UJ7
RMSD bond length	0.007
RMSD bond angle	0.791
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.500	3.660
High resolution limit [Å]	3.460	3.460
Rmerge	0.150	0.150
Number of reflections	11056
<I/σ(I)>	6	3.6
Completeness [%]	87.6	72.6
Redundancy	2.9	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1