5FTP
sulfur SAD phasing of Cdc23Nterm: data collection with a tailored X- ray beam size at 2.69 A wavelength (4.6 keV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 |
| Unit cell lengths | 61.249, 61.249, 151.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.250 - 3.100 |
| R-factor | 0.19046 |
| Rwork | 0.187 |
| R-free | 0.25963 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.397 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.250 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.110 | 0.720 |
| Number of reflections | 282255 | |
| <I/σ(I)> | 30.1 | 4.8 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 27.8 | 17.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | CRYSTALLIZATION DETAILS AS PER 3ZN3 |
