5FTF

Crystal structure of Pif1 helicase from Bacteroides double mutant L95C-I339C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	77
Detector technology	CCD
Collection date	2015-10-13
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	67.708, 73.482, 108.648
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.868 - 2.412
R-factor	0.2172
Rwork	0.213
R-free	0.29990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ftb
RMSD bond length	0.014
RMSD bond angle	1.346
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.870	2.500
High resolution limit [Å]	2.410	2.410
Rmerge	0.070	0.810
Number of reflections	19410
<I/σ(I)>	16.15	2.57
Completeness [%]	90.0	97
Redundancy	7.1	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1M BIS-TRIS PROPANE PH 6.5 0.1M CALCIUM ACETATE 10% PEG 4000