5FTE
Crystal structure of Pif1 helicase from Bacteroides in complex with ADP-AlF3 and ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2015-10-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 86.743, 86.743, 149.222 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.122 - 3.190 |
| R-factor | 0.2241 |
| Rwork | 0.221 |
| R-free | 0.27930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ftb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.732 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.120 | 3.300 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.110 | 1.060 |
| Number of reflections | 11303 | |
| <I/σ(I)> | 15.62 | 2.59 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 0.1M HEPES PH 7.1 3.5M SODIUM FORMATE 5% GLYCEROL 0.01M SPERMIDINE |
