5FTB

Crystal structure of Pif1 helicase from Bacteroides in complex with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	77
Detector technology	CCD
Collection date	2015-05-13
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	67.238, 74.930, 107.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.038 - 1.380
R-factor	0.1676
Rwork	0.167
R-free	0.18280
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	1.046
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.620	1.430
High resolution limit [Å]	1.380	1.380
Rmerge	0.060	0.970
Number of reflections	110840
<I/σ(I)>	18.6	2.39
Completeness [%]	99.0	1
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1M BIS-TRIS PROPANE PH 6.5 0.1M CALCIUM ACETATE 10% PEG 4000