5FTB
Crystal structure of Pif1 helicase from Bacteroides in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2015-05-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.238, 74.930, 107.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.038 - 1.380 |
| R-factor | 0.1676 |
| Rwork | 0.167 |
| R-free | 0.18280 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.046 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.620 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.060 | 0.970 |
| Number of reflections | 110840 | |
| <I/σ(I)> | 18.6 | 2.39 |
| Completeness [%] | 99.0 | 1 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BIS-TRIS PROPANE PH 6.5 0.1M CALCIUM ACETATE 10% PEG 4000 |
