5FT9

Arabidopsis thaliana nuclear protein-only RNase P 2 (PRORP2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-23
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 1
Unit cell lengths	70.500, 72.800, 80.300
Unit cell angles	63.10, 72.20, 78.40

Refinement procedure

Resolution	34.707 - 3.050
R-factor	0.2444
Rwork	0.242
R-free	0.28970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g26
RMSD bond length	0.005
RMSD bond angle	1.148
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.230
High resolution limit [Å]	3.050	3.050
Rmerge	0.120	1.500
Number of reflections	23923
<I/σ(I)>	8.7	0.8
Completeness [%]	92.4	98.2
Redundancy	3.5	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	277	PRORP2 STOCK SOLUTION WAS PREPARED AT 2.5 MG/ML IN 50 MM HEPES-NA PH 7.5, 250 MM NACL, 5%(W/V) GLYCEROL, 1 MM TCEP. PRORP2 WAS CRYSTALLIZED AT 277 K IN 2 MICROLITER MICROBATCH DROPS SET UP UNDER PARAFIN OIL BY MIXING 1 VOLUME OF PRORP2 SOLUTION WITH 1 VOLUME OF CRYSTALLANT SOLUTION CONTAINING 200 MM SODIUM MALONATE PH 6, 20% (W/V) PEG 3350.