5FSY
Crystal structure of Trypanosoma brucei macrodomain in complex with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.050, 54.620, 58.760 |
| Unit cell angles | 90.00, 95.72, 90.00 |
Refinement procedure
| Resolution | 19.490 - 1.700 |
| R-factor | 0.20279 |
| Rwork | 0.201 |
| R-free | 0.24384 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fsu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.743 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 1.110 |
| Number of reflections | 26185 | |
| <I/σ(I)> | 10.8 | 1.1 |
| Completeness [%] | 96.3 | 98.9 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.2 M SODIUM FLUORIDE, 0.1 M BIS-TRIS PROPANE PH 8.5, 20 % W/V PEG 3350 |
