5FSW
RNA dependent RNA polymerase QDE-1 from Thielavia terrestris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-28 |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.230, 165.950, 173.890 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 48.910 - 3.190 |
| R-factor | 0.21481 |
| Rwork | 0.213 |
| R-free | 0.25094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j7n |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.849 |
| Data reduction software | XDS |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.380 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.170 | 0.410 |
| Number of reflections | 78767 | |
| <I/σ(I)> | 8.23 | 3.14 |
| Completeness [%] | 99.2 | 96.4 |
| Redundancy | 3.47 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 100 MM TRIS PH8, 75 MM NACL, 10% PEG10K |
