5FSH
Crystal structure of Thermus thermophilus Csm6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 95.309, 207.070, 58.903 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.701 - 2.301 |
| R-factor | 0.2202 |
| Rwork | 0.219 |
| R-free | 0.24380 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.784 |
| Data reduction software | SLS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.950 |
| Number of reflections | 98111 | |
| <I/σ(I)> | 17.9 | 1.5 |
| Completeness [%] | 98.0 | 87.8 |
| Redundancy | 6.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 14% (W/V) PEG 3400, 35 MM NICL2 |
