5FSB
Structure of tectonin 2 from laccaria bicolor in complex with 2-o-methyl-methyl-seleno-beta-l-fucopyranoside
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-16 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 69.058, 69.058, 349.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.530 - 1.650 |
| R-factor | 0.13474 |
| Rwork | 0.133 |
| R-free | 0.16656 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.530 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.090 | 0.430 |
| Number of reflections | 39383 | |
| <I/σ(I)> | 19.5 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.6M MAGNESIUM SULFATE 0.1M MES PH 6.5. 80% LITHIUM SULFATE WAS USED AS CRYOPROTECTANT |
