5FS4
Bacteriophage AP205 coat protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.600, 114.930, 42.800 |
| Unit cell angles | 90.00, 98.14, 90.00 |
Refinement procedure
| Resolution | 57.530 - 1.730 |
| R-factor | 0.23246 |
| Rwork | 0.230 |
| R-free | 0.27454 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.832 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BUCCANEER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.450 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.170 | 0.630 |
| Number of reflections | 28660 | |
| <I/σ(I)> | 4.6 | 1.6 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 20 % ETHYLENE GLYCOL AQUEOUS SOLUTION, 7 MG/ML PROTEIN IN 40 MM TRIS-HCL PH 8,0, 300 MM NACL, VAPOR DIFFUSION SITTING DROP METHOR, 0.7 MIKROL PRECIPITANT PLUS 1 MIKROL PROTEIN,TEMPERATURE 294 K |
