5FRV

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR in complex with 4-methylphenol (Cresol)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	287
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	46.696, 70.491, 105.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.348 - 1.900
R-factor	0.2284
Rwork	0.227
R-free	0.27190
Structure solution method	MIR
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.086
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.080
Number of reflections	56079
<I/σ(I)>	37.29	0.43
Completeness [%]	99.4	99.7
Redundancy	13.7	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1