5FRQ
Crystal Structure of Helicobacter pylori beta clamp bound to DNA ligase peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 289 |
| Detector technology | CCD |
| Collection date | 2015-11-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.829, 146.147, 179.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.230 - 2.900 |
| R-factor | 0.23302 |
| Rwork | 0.231 |
| R-free | 0.26154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rki |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.807 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 35997 | |
| <I/σ(I)> | 11 | 1.9 |
| Completeness [%] | 94.3 | 95 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6% W/V PEG 20,000, 20% V/V PEG MME550, 0.01M MGCL2, 0.1M MOPS/HEPES-NA PH7.3, 0.2M AMMONIUM CITRATE |
