5FRK
SeMet crystal structure of Erwinia amylovora AmyR amylovoran repressor, a member of the YbjN protein family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 138.028, 49.618, 57.813 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.010 - 2.120 |
| R-factor | 0.21887 |
| Rwork | 0.216 |
| R-free | 0.27584 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.778 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.340 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.090 | 1.000 |
| Number of reflections | 22681 | |
| <I/σ(I)> | 24 | 1.4 |
| Completeness [%] | 97.2 | 75.4 |
| Redundancy | 12.9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 293 | 0.1 M SODIUM CACODYLATE PH7.3 0.3 M MAGNESIUM CHLORIDE, 42% PEG 200, 293 K PROTEIN CONCENTRATION 8 MG/ML |
