5FR7

Erwinia amylovora AmyR amylovoran repressor, a member of the YbjN protein family

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	137.163, 49.816, 57.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.260 - 1.950
R-factor	0.22588
Rwork	0.224
R-free	0.26863
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SPDB ENTRY 5FRK
RMSD bond length	0.016
RMSD bond angle	1.797
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.260	2.140
High resolution limit [Å]	1.950	1.950
Rmerge	0.070	1.010
Number of reflections	29587
<I/σ(I)>	9.2	1.8
Completeness [%]	99.7	99.6
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.9		0.1M SODIUM CACODYLATE PH 6.9, 0.2M MAGNESIUM CHLORIDE, 46% (V/V) PEG200