5FR6
The structure of polycomb ULD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-06-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 78.275, 78.275, 43.119 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.140 - 2.510 |
| R-factor | 0.23748 |
| Rwork | 0.233 |
| R-free | 0.32833 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hpm |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.783 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.090 | 0.480 |
| Number of reflections | 5292 | |
| <I/σ(I)> | 33.93 | 3.27 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 10 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100MM MES, PH 6.5, 50MM MGCL2, 7% ISOPROPANOL, 6% PEG 4000 |
