5FQ4
Crystal structure of the lipoprotein BT2263 from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.757, 80.025, 120.047 |
| Unit cell angles | 90.00, 100.12, 90.00 |
Refinement procedure
| Resolution | 47.535 - 1.900 |
| R-factor | 0.1593 |
| Rwork | 0.158 |
| R-free | 0.19870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q69 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.802 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.750 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.840 |
| Number of reflections | 81475 | |
| <I/σ(I)> | 7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM ACETATE TRIHYDRATE 0.1 M TRIS PH 8.5 30% V/V PEG4000 |
