5FQ3
Crystal structure of the lipoprotein BT2262 from Bacteroides thetaiotaomicron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 167.320, 167.320, 100.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.749 - 3.100 |
| R-factor | 0.204 |
| Rwork | 0.199 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | N-TERMINAL DOMAIN FROM COMPLEX STRUCTURE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.180 | 1.500 |
| Number of reflections | 26439 | |
| <I/σ(I)> | 15.3 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M AMMONIUM SULPHATE 0.1 M SODIUM HEPES PH 7.5 2% W/W PEG400 |
