5FQ2

Crystal structure of human SUMO E1 UFD domain in complex with Ubc9 in a P422 space group.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 43 21 2
Unit cell lengths	129.614, 129.614, 66.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.439 - 2.201
R-factor	0.2303
Rwork	0.229
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.114
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.440	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.040	0.600
Number of reflections	29309
<I/σ(I)>	26.4	4.3
Completeness [%]	99.8	98.4
Redundancy	10.9	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1